Evaluation of a duplex real-time PCR in human serum for simultaneous detection and differentiation of Schistosoma mansoni and Schistosoma haematobium infections – cross-sectional study
نویسندگان
چکیده
We evaluated a one-tube multiplex real-time PCR targeting DNA of Schistosoma haematobium complex and S. mansoni in serum samples obtained at different German diagnostic centers. Simplex protocols for the detection multi-copy DNA-repeats Dra1 Sm1-7 were combined to new format. The was subjected full validation including evaluation real-life setting with travelers migrants. results compared those stool urine microscopy, serology, circulating cathodic antigen (CCA) rapid tests urine. Sensitivity specificity approaches analyzed using latent class analysis (LCA). LCA assessment indicated sensitivity 94.9% 98.4%, respectively, if serology included calculation, 100% 95.6%, not as parameter necessarily associated active infection. Agreement between procedures genus level fair (kappa 0.273) moderate 0.420) included. assay proved be highly reliable diagnosis schistosomiasis
منابع مشابه
Detection of Schistosoma mansoni and Schistosoma haematobium by Real-Time PCR with High Resolution Melting Analysis
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ژورنال
عنوان ژورنال: Travel Medicine and Infectious Disease
سال: 2021
ISSN: ['1873-0442', '1477-8939']
DOI: https://doi.org/10.1016/j.tmaid.2021.102035